An experimental model for osteosarcoma was developed in which, after
Moloney murine sarcoma virus was injected into the tibial marrow space of
three strains of inbred neonatal rats, a highly malignant neoplasm arose
within ten days. This tumor was readily maintained in tissue culture and
was transplantable to adult rats. It arose in the metaphyseal marrow of
several bones, was locally invasive, metastasized to the lungs, and
histologically resembled osteosarcoma.