A transplantable murine osteosarcoma is described. Following
transplantation into a syngeneic mouse the tumor grows rapidly and kills
the mouse with pulmonary metastases simulating human osteosarcoma. A
cell-mediated antibody response is evoked in the host mouse as demonstrated
by in vivo and in vitro tests. The number of pulmonary metastases may be
decreased with adjunctive immunotherapy following excision of the primary
tumor. Immunotherapeutic materials include BCG and isologous cells treated
with Vibrio cholerae neuraminidase.