The concentrations in serum, cancellous bone, and cortical bone of three
antibiotics--nafcillin, cefazolin, and gentamicin--were measured in eleven
normal dogs. All antibiotics rapidly penetrated into bone. The peak
concentration in serum was reached within twenty minutes of intravenous
injections, and the concentration in bone generally paralleled that in
serum. Cancellous bone had higher concentrations than did cortical bone
because of the admixture of blood.