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Morphological, Immunochemical, and Biochemical Study of Rabbit Achilles Tendon at Various Ages
Ernesto Ippolito, M.D.; Pier Giorgio Natali, M.D.; Franco Postacchini, M.D.; Lidia Accinni, PH.D.; Cesare De Martino, M.D.
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ROME, ITALY
II Department of Orthopaedic Surgery, Università degli Studi, Piazzale delle Scienze, Rome, Italy.
Regina Elena Institute for Cancer Research, Rome, Italy.
C.N.R. Clinica Medica II, University of Rome, Rome, Italy.

The Journal of Bone and Joint Surgery, Incorporated
The Journal of Bone & Joint Surgery.  1980; 62:583-598 
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Abstract

With aging, rabbit tendon tissue undergoes a series of morphological and biochemical changes which involve both the cells and the extracellular matrix. The extracellular matrix increases in volume, causing a relative decrease of the number of cells per unit of tissue surface. The tenoblasts become longer and more slender, while their cytoplasmic processes increase in number and become thinner and more elongated, forming a dense network. In addition, tendon cells show a marked decrease in the intracytoplasmic organelles responsible for protein synthesis, while their intracellular content of contractile proteins does not change.

With aging, collagen fibers increase in diameter and vary more in thickness. These morphological changes correspond to biochemical changes that include an increase in collagen, a decrease in mucopolysaccharides, and a decrease in water content. During aging parallel changes occur in the elastic fibers, which decrease in number and show structural alterations.

Clinical Relevance: Ultrastructural and biochemical studies of tendon diseases need a normal comparison. Our ultrastructural and biochemical findings in aging tendon may be useful in that regard. The presence of actin and myosin in tendon cells could be related to some aspects of tendon physiology and pathology.

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    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
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