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Evaluation of Preoperative Cultures Before Second-Stage Reimplantation of a Total Knee Prosthesis Complicated by Infection A Comparison-Group Study*
Michael A. Mont, M.D.†; Barry J. Waldman, M.D.‡; David S. Hungerford, M.D.§
View Disclosures and Other Information
Investigation performed at the Division of Arthritis Surgery, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland
*No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article. No funds were received in support of this study.
†Sinai Hospital of Baltimore, 2401 West Belvedere Avenue, Baltimore, Maryland 21215-5271. E-mail address: rhondamont@aol.com.
‡Orthopaedic Specialty Center, 6080 Falls Road, Suite 203, Baltimore, Maryland 21209. E-mail address: bwaldman@mdorthoteam.com.
§Division of Arthritis Surgery, Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Good Samaritan Professional Building, Suite G-1, 5601 Loch Raven Boulevard, Baltimore, Maryland 21239. E-mail address: dhunger@welchlink.welch. jhu.edu.

The Journal of Bone & Joint Surgery.  2000; 82:1552-1552 
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Abstract

Background: Two-stage reimplantation has proven to be highly successful in the treatment of patients with infection at the site of a total knee arthroplasty. However, up to 20 percent of patients have a recurrence of infection following this treatment. The purpose of our study was to determine whether aspiration of the affected joint and culture of the specimen, performed before reimplantation and after discontinuation of antibiotic therapy, would help to identify patients who might have a recurrent infection.

Methods: We prospectively followed sixty-nine patients who were treated for a culture-proven deep infection at the site of a total knee arthroplasty. Group I consisted of thirty-five patients who were treated with removal of the prosthetic components and irrigation and d衲idement of the joint, followed by six weeks of antibiotic therapy and reimplantation of a prosthesis. Group II was composed of thirty-four patients who were treated with removal of the components and irrigation and d衲idement of the joint, six weeks of antibiotic therapy, and then repeat culture four weeks after the antibiotic course had ended. If the culture was negative, the patient was managed with a second-stage reimplantation of a prosthesis. If the culture was positive, the protocol was repeated, beginning with irrigation and d衲idement. The two groups were similar with regard to male-to-female ratio, age, preoperative Knee Society scores, time since primary surgery, types of infectious organisms, duration of symptoms, duration of follow-up, and number of previous revisions. All of the patients were evaluated clinically with use of the objective scoring system of the Knee Society and were followed with serial radiographs. Success was defined as no infection and a functional prosthesis, with a Knee Society score of at least 75 points at the last (thirty-six-month-minimum) follow-up evaluation.

Results: Of the thirty-five patients in Group I, five (14 percent) had recurrence of infection. One of the patients was managed with a successful second-stage revision, three were managed with arthrodesis of the knee, and one continued dwith chronic antibiotic suppressive treatment. Of the thirty-four patients in Group II, three (9 percent) had a positive culture after the course of antibiotics. The protocol was repeated for all three, and they subsequently had a successful second revision. One other patient (3 percent) in Group II, who had a negative culture, had a recurrent infection and was eventually managed with arthrodesis of the knee.

Conclusions: Prerevision cultures, grown after discontinuation of antibiotic treatment and before reimplantation of the components, helped to identify the patients with infection at the site of a total knee arthroplasty in whom the infection might recur. The performance of aspiration and cultures resulted in a substantial improvement in the clinical outcome.

Figures in this Article
    Two-stage revision arthroplasty1-3,9,12,17,20,26,28 appears to be the most successful treatment for late hematogenous, deep infection. The first stage consists of irrigation and d衲idement of the infected joint with removal of the prosthesis. Often, an antibiotic-loaded cement spacer is used to increase available levels of antibiotics and to prevent contracture of the joint space. The patient is subsequently treated with a course of intravenous antibiotics (usually six weeks) and then is returned to the operating room for implantation of the prosthesis. This strategy has proven reliable in the treatment of infections at the sites of total knee arthroplasties, with successful eradication of 80 to 100 percent of the infections in series of twenty to sixty patients15,17,26,28. Despite this success, between 3 and 20 percent of patients managed with two-stage revision have a recurrence of the infection. In most series reported in the literature, the prosthesis was reimplanted without the performance of additional cultures of specimens from the joint before the discontinuation of antibiotic therapy26,28.
    In our study, we examined the role of preoperative cultures performed at least four weeks after antibiotic therapy had been discontinued. Patients with positive cultures were treated with more irrigation and d衲idement and a second course of antibiotics; revision was performed only when preoperative cultures were negative. The goal of our study was to see whether this approach reduced the morbidity and increased the successful outcome of treatment of infection at the site of a total knee arthroplasty when compared with a standard method of two-stage reimplantation without culture before the reimplantation.
     
    Anchor for JumpAnchor for JumpTable I:  Comparison of Postoperative Parameters of the Two Treatment Groups*
    *Culture of the aspirate and culture of the affected joint were performed before reimplantation and after discontinuation of antibiotic therapy in Group II but not in Group I.†NS = not significant.‡The values are given as the mean, with the range in parentheses.§Three patients had a recurrent infection before reimplantation, and the joints were debrided.
    ParameterGroup I (N = 35)Group II (N = 34)P Value†
    Duration of follow-up‡ (mos.)      68 (36-114)    58 (36-91)NS
    No. of patients with recurrent infection        5 (14%)      1 (3%)§<0.05
    No. of procedures per patient2.7 (2-4)2.8 (2-4)NS
    No. of patients with Knee Society score 75 points      32 (91%)        33 (97%)NS
    Knee Society score of infection-free knees‡ (points)      86 (80-95)        88 (64-98)NS
     
    Anchor for JumpAnchor for JumpTable II:  Patient Data for Group I
    CaseGender, Age at Infection (yrs.)Time from Index Op. to Diagnosis (mos.)Type of ArthroplastyFindings on CultureDurat. of Symptoms Prior to Diagnosis(days)OutcomeKnee Society Score at Final Follow-up(points)Durat. of Follow-up (mos.)
      1M, 6437PrimaryS. aureus  54Success84  60
      2F, 6242PrimaryStrep. pneumoniae    1Success87  48
      3F, 5823Primaryb-hemolytic strep.  24Success80  56
      4F, 56  2Primaryb-hemolytic strep.    4Failure64  36
      5M, 46  6PrimaryS. aureus  77Success80  40
      6F, 5923PrimaryS. aureus  45Success93  65
      7M, 6322PrimaryS. aureus  78Failure82  51
      8F, 60  8PrimaryS. aureus  44Success80  92
      9M, 58  5RevisionE. coli  53Success86  60
    10M, 6424PrimaryS. aureus  22Success94  92
    11M, 57  4PrimaryMixed gram-negative organisms  49Success90  61
    12F, 6283PrimaryStrep. viridans    8Failure58  69
    13M, 5548PrimaryS. epidermidis  79Success81  70
    14F, 7443RevisionS. aureus  47Success90  69
    15M, 6761PrimaryS. epidermidis  36Success80  56
    16F, 6840PrimaryS. aureus130Success94  57
    17M, 7549PrimaryS. aureus  18Success85  68
    18F, 6497PrimaryP. aeruginosa  74Success84  89
    19M, 7624PrimaryS. aureus138Success86  60
    20F, 6363PrimaryEnterococcus    9Failure61  57
    21M, 6635PrimaryS. epidermidis120Success81  85
    22M, 7044RevisionS. aureus  53Success80114
    23F, 8061PrimaryK. oxytoca    8Success92  70
    24M, 7122PrimaryS. aureus  62Failure95  88
    25M, 6620PrimaryS. aureus  49Success95  69
    26F, 65  6PrimaryS. aureus  80Success90  66
    27M, 7112RevisionS. aureus    9Success81  86
    28F, 7242PrimaryS. epidermidis  20Success87  96
    29M, 7329PrimaryS. epidermidis  49Success85  92
    30F, 6418PrimaryP. mirabilis  76Success83  84
    31M, 65  3PrimaryS. aureus  10Success88  47
    32F, 5233PrimaryS. epidermidis  51Success89  52
    33F, 5363PrimaryS. aureus  20Success87  68
    34F, 5417PrimaryS. aureus  19Success90  44
    35F, 6546PrimaryS. epidermidis  50Success83  64
     
    Anchor for JumpAnchor for JumpTable III:  Patient Data for Group II
    Case Gender, Age at Infection (yrs.)Time from Index Op. to Diagnosis (mos.)Type of Arthroplasty Findings on Culture Durat. of Symptoms Prior to Diagnosis (days)Outcome Knee Society Score at Final Follow-up (points)Durat. of Follow- up (mos.)
      1M, 7034PrimaryS. epidermidis  29Success8458
      2M, 6760PrimaryS. epidermidis  30Success8548
      3F, 62  1PrimaryS. aureus    4Success8768
      4F, 56  2PrimaryP. mirabilis  15Success8460
      5M, 6346RevisionS. epidermidis  16Success9191
      6F, 6536PrimaryS. aureus    3Success9367
      7F, 6422PrimaryS. aureus  62Failure6448
      8M, 6174PrimaryS. epidermidis  30Success9236
      9F, 60  4RevisionS. aureus  50Success8962
    10M, 6927PrimaryS. aureus160Success8658
    11M, 5854PrimaryS. epidermidis  20Success7669
    12M, 6824PrimaryS. aureus  64Success8956
    13F, 6830PrimaryMixed gram-negative organisms    4Success9036
    14M, 59  6PrimaryS. aureus  60Success8768
    15M, 6923PrimaryStrep. viridans  52Success9060
    16F, 70  3PrimaryS. aureus  58Success9076
    17M, 7015RevisionK. oxytoca  83Success9159
    18F, 5976PrimaryS. aureus108Success7759
    19M, 6420PrimaryS. aureus  82Success8790
    20F, 8080PrimaryS. epidermidis322Success9758
    21M,77  2PrimaryS. aureus  20Success9474
    22F, 8027PrimaryS. aureus  14Success9557
    23F, 7164PrimaryS. epidermidis  54Success7836
    24M, 7636RevisionGroup-B b-hemolytic strep.  98Success8548
    25F, 8224PrimaryS. aureus  86Success9060
    26M, 7976PrimaryS. aureus  29Success8055
    27F, 6936PrimaryS. epidermidis    4Success9559
    28F, 7848PrimaryS. aureus  20Success9536
    29F, 7262PrimaryStrep. viridans  85Success8456
    30M, 7534PrimaryE. coli  86Success9878
    31F, 7450PrimaryS. aureus    3Success8060
    32F, 6840PrimaryS. epidermidis  48Success8036
    33M, 6934PrimaryP. aeruginosa  89Success8857
    34F, 74  9PrimaryS. aureus  30Success8948
    Sixty-nine consecutive patients with a deep infection following total knee arthroplasty that was treated with two-stage revision arthroplasty between January 1, 1989, and December 31, 1993, were prospectively followed. The deep infections developed from one to ninety-seven months after the index knee arthroplasty. Demographic information such as preoperative history, operative findings, and postoperative course were obtained from hospital and outpatient records and recorded. In addition, preoperative and postoperative radiographs, nuclear medicine scans, laboratory findings, and culture results were reviewed. Anteroposterior and lateral radiographs were made annually to check for progressive radiolucencies or migration of the prosthesis. The patients were divided into two groups depending on the treatment protocol, which was selected solely on the basis of the preference of one of the two senior surgeons (M. A. M. or D. S. H.) and not on the basis of the severity of the infection or any other factors. The patients in Group I were managed by one surgeon, and the patients in Group II were managed by the other surgeon. The objective clinical rating system of the Knee Society14 was used to follow the patient's clinical course, and the radiographic scoring system of the Knee Society6 was used to assess postoperative radiographs.

    Study Groups

    Group I consisted of thirty-five knees treated with removal of the components, irrigation and d衲idement of the joint, six weeks of intravenous antibiotics, and reimplantation of a total knee prosthesis. Group II was composed of thirty-four knees treated with removal of the components, irrigation and d衲idement, six weeks of intravenous antibiotics, and cultures of material obtained from one knee-joint aspiration performed four weeks after completion of the antibiotic therapy. If the culture was negative after two weeks, the patient underwent reimplantation. If the culture was positive, the patient was managed with repeat irrigation and d衲idement of the joint and then was reentered into the protocol. The operative procedure was similar for all of the patients. The joint was extensively debrided of all grossly infected tissue; the d衲idement included a complete synovectomy. The components were removed, and the bone surfaces were aggressively curetted. A cement spacer impregnated with two grams of tobramycin (one gram per pack of cement) was placed loosely into the joint, and the wound was closed over two suction drains. For all of the patients, intravenous antibiotics were chosen after consultation with the infectious disease service. Antibiotic levels were carefully monitored, and a bactericidal titer of at least 1:8 was maintained for the six-week course. As mentioned, the treatment protocol was determined preoperatively solely by the preference of the attending surgeon.

    Demographics

    Group I consisted of seventeen men and eighteen women. The mean age of the patients at the time of infection was sixty-four years (range, forty-six to eighty years). The mean time from the index arthroplasty until the diagnosis of infection was thirty-three months (range, two to ninety-seven months). The mean duration of symptoms until diagnosis was forty-eight days (range, one to 138 days). There were thirty-one primary and four revision arthroplasties. All knees had a late deep infection proven on culture. The mean duration of follow-up was sixty-eight months (range, thirty-six to 114 months).
    Group II consisted of sixteen men and eighteen women. The mean age of the patients at the time of infection was sixty-nine years (range, fifty-six to eighty-two years). The mean time from the index arthroplasty until the diagnosis of infection was thirty-five months (range, one to eighty months). The mean duration of symptoms until the diagnosis was fifty-six days (range, four to 322 days). There were thirty primary and four revision arthroplasties. All knees had a late deep infection proven on culture. The mean duration of follow-up was fifty-eight months (range, thirty-six to ninety-one months).
    There was no significant difference between the two groups in terms of the male:female ratio, age, preoperative Knee Society score, time from the index arthroplasty until the diagnosis of infection, types of infectious organisms, duration of symptoms before diagnosis, number of previous revisions, or duration of follow-up (p > 0.05).

    Treatment

    At the time of reimplantation, all patients had careful d衲idement of the affected joint, removal of the cement spacer, and reinsertion of a knee prosthesis. All prostheses were fixed with cement impregnated with antibiotics (tobramycin, one gram per pack). If a patient was found to be free of infection at the time of reimplantation (with negative gram stains and specimens that were completely free of polymorphonuclear leukocytes), no antibiotics were given. If a knee was found to have a persistent infection (polymorphonuclear leukocyte counts of ten or more cells per high-power field), it was debrided again, another course of antibiotics was given, and the patient was reentered into the protocol. The patients were followed with yearly physical and radiographic examinations, and data from the most recent follow-up evaluation were recorded as the final results in this report. The reimplantation was considered a success when the objective Knee Society score was 75 points or more and the patient was free of infection at the time of the most recent follow-up.

    Data Analysis

    The primary issue was whether performing preoperative aspiration and culture had a significant effect on the rates of reinfection and successful clinical outcomes following two-stage revision arthroplasty. The null hypothesis suggested that performing preoperative aspiration and culture would have no effect on the rate of reinfection or on the final clinical outcome. Differences in frequencies of infection between Groups I and II were analyzed with the chi-square test. Differences in Knee Society scores were analyzed with the two-tailed Student t test.

    Group I (Tables I and II)

    Of the thirty-five patients in Group I, thirty (86 percent) had no recurrence of infection. These patients all had a well functioning prosthesis and were free of infection at the most recent follow-up evaluation. Of the five patients (14 percent) who had a recurrence of infection, one was managed with repeat delayed exchange arthroplasty. At the time of the last (fifty-one-month) follow-up, this patient had retained the second revision prosthesis, was free of apparent infection, and had a Knee Society score of 82 points. Three of the other patients who had recurrent infection had successful arthrodesis of the knee24. One patient continued with chronic antibiotic suppression and did not undergo removal of the revision prosthesis. The mean time to reinfection was four months (range, two to six months). All reinfections were from the organism that had caused the initial infection, although in three of the five patients the sensitivities of the organism to antibiotics had changed. The mean postoperative Knee Society score associated with successful clinical outcomes was 86 points (range, 80 to 95 points). The mean knee flexion at the time of the latest follow-up in the patients with initial eradication of the infection was 95 degrees (range, 30 to 122 degrees), and the mean extension was -5 degrees (range, 0 to -10 degrees).

    Group II (Tables I and III)

    Of the thirty-four patients in Group II, thirty-one (91 percent) had a negative culture after the course of antibiotics had ended. These patients were managed with second-stage total knee arthroplasty after the negative culture was obtained. Thirty of the thirty-one patients were free of infection and had a functioning prosthesis at the time of the last follow-up. One patient had a recurrent infection after the second-stage revision; the infection was caused by Staphylococcus aureus; this was different from the initial infecting organism, which was a streptococcus species. This patient eventually had a knee arthrodesis24. The three patients (9 percent) who had a positive culture were managed with six weeks of intravenous antibiotics after more operative d衲idement. All three had the same infecting organisms as they had had preoperatively, although the sensitivities to antibiotics had changed. The three patients subsequently had a negative culture and a successful revision arthroplasty.
    The mean postoperative Knee Society score for all of the knees in Group II was 87 points (range, 64 to 98 points). At the time of the final follow-up, the mean knee flexion in the patients with initial eradication of the infection was 98 degrees (range, 63 to 130 degrees), and the mean extension was -4 degrees (range, 0 to -12 degrees).
    There were no signs of loosening, on the most recent postoperative radiographs, of any of the surviving prostheses in either group. No prosthesis had migrated compared with the position seen on postoperative radiographs. Three revision femoral components showed partial, nonprogressive radiolucent lines anteriorly (in zone 1) on the lateral radiograph. Two prostheses had partial, nonprogressive radiolucent lines under the medial tibial tray (in zones 1 and 2) on the anteroposterior radiograph.
    The optimum treatment for an infection complicating total knee arthroplasty continues to be controversial. An ideal treatment would entail a minimum of surgical procedures, an acceptable level of joint function during the treatment, and a high rate of success. Each method represents a tradeoff between the success rate and the morbidity of the treatment itself. Protocols that include retention of the affected components seek to reduce the need for additional surgical treatment and the period of disability. These procedures include arthroscopic irrigation and d衲idement8,25, open irrigation and d衲idement5,18,22,23, as well as one-stage revision arthroplasty7,10,11. Unfortunately, the high rates of reinfection seen with these methods have been disappointing.
    Two-stage revision arthroplasty is the most commonly reported alternative. This method involves removal of the prosthesis followed by at least six weeks of antibiotics. Studies of two-stage revision have demonstrated the most consistent and successful results. Windsor et al.28 reported a success rate of 97 percent (thirty-two) of the thirty-three knees treated. Borden and Gearen4 reported success in ten of eleven knees treated with two-stage revision. Even with these uniformly successful results, reinfections do occur. The most likely source of reinfection is bacteria retained in the joint following the first-stage d衲idement. Bacterial resistance, inadequate d衲idement, or a new infection may account for persistence of infection in some cases.

    Rationale for Prerevision Cultures

    Despite the superior clinical results obtained with two-stage arthroplasty in a number of centers, there may be modifications that can improve this method. Preoperative cultures may be a sensitive way of detecting persistent infection. A number of recommendations have been made regarding these cultures16. Insall et al.13 recommended that antibiotic therapy be stopped and then cultures be performed before revision arthroplasty. Other authors have recommended that cultures be performed while the patient is receiving antibiotic therapy or have believed that there should be no aspiration at all before a revision procedure26-29. Any joint that becomes reinfected following revision may have harbored bacteria that were suppressed by the antibiotic course. These joints show few or no clinical signs of continued infection, and intraoperative sampling may not be helpful because of low bacterial counts. Allowing four weeks off antibiotics before culture of specimens from the joint gives any bacteria present an opportunity to proliferate and potentially to be detected by culture of the aspirate. This technique also allows a period for clinical symptoms from bacterial proliferation, if present, to manifest themselves. The disadvantage of repeat cultures is a delay in definitive operative treatment as well as the prolonged morbidity associated with a minimally functioning knee joint. Many patients with an infection at the site of a prosthesis are often those who are least able to tolerate a long period of relative immobility19,21. In the current study, there were no significant differences between groups with regard to the most recent follow-up scores of the knees with successful reimplantation; this finding indicates that the delay in treatment had no long-term effects.
    One weakness in our study is the lack of randomization of the patients. The patients were managed according to the protocol of the two senior surgeons, and differences in their approaches may account for the reported results. However, all of the patients were managed with similar operative techniques, operating-room teams, equipment, and assistant surgeons. Since all six recurrences happened after less than one year of follow-up, the minimum three-year follow-up used in this study should have been adequate to detect all persistently infected joints.
    Two-stage revision arthroplasty was found to be an effective method for treating infection after total knee arthroplasty. This study suggests that prerevision cultures, after the discontinuation of antibiotic treatment, should be performed in all patients treated with this procedure. With identification of continuing infection, the morbidity associated with recurrent infection may be avoided and the number of surgical procedures may be reduced.
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    Anchor for JumpAnchor for JumpTable I:  Comparison of Postoperative Parameters of the Two Treatment Groups*
    *Culture of the aspirate and culture of the affected joint were performed before reimplantation and after discontinuation of antibiotic therapy in Group II but not in Group I.†NS = not significant.‡The values are given as the mean, with the range in parentheses.§Three patients had a recurrent infection before reimplantation, and the joints were debrided.
    ParameterGroup I (N = 35)Group II (N = 34)P Value†
    Duration of follow-up‡ (mos.)      68 (36-114)    58 (36-91)NS
    No. of patients with recurrent infection        5 (14%)      1 (3%)§<0.05
    No. of procedures per patient2.7 (2-4)2.8 (2-4)NS
    No. of patients with Knee Society score 75 points      32 (91%)        33 (97%)NS
    Knee Society score of infection-free knees‡ (points)      86 (80-95)        88 (64-98)NS
    Anchor for JumpAnchor for JumpTable II:  Patient Data for Group I
    CaseGender, Age at Infection (yrs.)Time from Index Op. to Diagnosis (mos.)Type of ArthroplastyFindings on CultureDurat. of Symptoms Prior to Diagnosis(days)OutcomeKnee Society Score at Final Follow-up(points)Durat. of Follow-up (mos.)
      1M, 6437PrimaryS. aureus  54Success84  60
      2F, 6242PrimaryStrep. pneumoniae    1Success87  48
      3F, 5823Primaryb-hemolytic strep.  24Success80  56
      4F, 56  2Primaryb-hemolytic strep.    4Failure64  36
      5M, 46  6PrimaryS. aureus  77Success80  40
      6F, 5923PrimaryS. aureus  45Success93  65
      7M, 6322PrimaryS. aureus  78Failure82  51
      8F, 60  8PrimaryS. aureus  44Success80  92
      9M, 58  5RevisionE. coli  53Success86  60
    10M, 6424PrimaryS. aureus  22Success94  92
    11M, 57  4PrimaryMixed gram-negative organisms  49Success90  61
    12F, 6283PrimaryStrep. viridans    8Failure58  69
    13M, 5548PrimaryS. epidermidis  79Success81  70
    14F, 7443RevisionS. aureus  47Success90  69
    15M, 6761PrimaryS. epidermidis  36Success80  56
    16F, 6840PrimaryS. aureus130Success94  57
    17M, 7549PrimaryS. aureus  18Success85  68
    18F, 6497PrimaryP. aeruginosa  74Success84  89
    19M, 7624PrimaryS. aureus138Success86  60
    20F, 6363PrimaryEnterococcus    9Failure61  57
    21M, 6635PrimaryS. epidermidis120Success81  85
    22M, 7044RevisionS. aureus  53Success80114
    23F, 8061PrimaryK. oxytoca    8Success92  70
    24M, 7122PrimaryS. aureus  62Failure95  88
    25M, 6620PrimaryS. aureus  49Success95  69
    26F, 65  6PrimaryS. aureus  80Success90  66
    27M, 7112RevisionS. aureus    9Success81  86
    28F, 7242PrimaryS. epidermidis  20Success87  96
    29M, 7329PrimaryS. epidermidis  49Success85  92
    30F, 6418PrimaryP. mirabilis  76Success83  84
    31M, 65  3PrimaryS. aureus  10Success88  47
    32F, 5233PrimaryS. epidermidis  51Success89  52
    33F, 5363PrimaryS. aureus  20Success87  68
    34F, 5417PrimaryS. aureus  19Success90  44
    35F, 6546PrimaryS. epidermidis  50Success83  64
    Anchor for JumpAnchor for JumpTable III:  Patient Data for Group II
    Case Gender, Age at Infection (yrs.)Time from Index Op. to Diagnosis (mos.)Type of Arthroplasty Findings on Culture Durat. of Symptoms Prior to Diagnosis (days)Outcome Knee Society Score at Final Follow-up (points)Durat. of Follow- up (mos.)
      1M, 7034PrimaryS. epidermidis  29Success8458
      2M, 6760PrimaryS. epidermidis  30Success8548
      3F, 62  1PrimaryS. aureus    4Success8768
      4F, 56  2PrimaryP. mirabilis  15Success8460
      5M, 6346RevisionS. epidermidis  16Success9191
      6F, 6536PrimaryS. aureus    3Success9367
      7F, 6422PrimaryS. aureus  62Failure6448
      8M, 6174PrimaryS. epidermidis  30Success9236
      9F, 60  4RevisionS. aureus  50Success8962
    10M, 6927PrimaryS. aureus160Success8658
    11M, 5854PrimaryS. epidermidis  20Success7669
    12M, 6824PrimaryS. aureus  64Success8956
    13F, 6830PrimaryMixed gram-negative organisms    4Success9036
    14M, 59  6PrimaryS. aureus  60Success8768
    15M, 6923PrimaryStrep. viridans  52Success9060
    16F, 70  3PrimaryS. aureus  58Success9076
    17M, 7015RevisionK. oxytoca  83Success9159
    18F, 5976PrimaryS. aureus108Success7759
    19M, 6420PrimaryS. aureus  82Success8790
    20F, 8080PrimaryS. epidermidis322Success9758
    21M,77  2PrimaryS. aureus  20Success9474
    22F, 8027PrimaryS. aureus  14Success9557
    23F, 7164PrimaryS. epidermidis  54Success7836
    24M, 7636RevisionGroup-B b-hemolytic strep.  98Success8548
    25F, 8224PrimaryS. aureus  86Success9060
    26M, 7976PrimaryS. aureus  29Success8055
    27F, 6936PrimaryS. epidermidis    4Success9559
    28F, 7848PrimaryS. aureus  20Success9536
    29F, 7262PrimaryStrep. viridans  85Success8456
    30M, 7534PrimaryE. coli  86Success9878
    31F, 7450PrimaryS. aureus    3Success8060
    32F, 6840PrimaryS. epidermidis  48Success8036
    33M, 6934PrimaryP. aeruginosa  89Success8857
    34F, 74  9PrimaryS. aureus  30Success8948
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